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FeCV table

Feline calicivirus

Far-UV Sterilray™ commissioned a virology lab in January 2007 to conduct a screening test for Sterilray's efficacy against the norovirus using Feline calicivirus (FeCV) as the surrogate. 52 mj/cm2 irradiance was used on the 104 and 106 virus amounts. 101 mj/cm2 was used on the 108 amount. The results of this test show that Sterilray is as effective killing this virus as it is with various bacteria. Click on comparison test data for more information.
The 1.2 log limit of detection is based on the standard 1 log dilution intervals used in this test. Future tests will not dilute the treated virus culture that will be used as the innoculant. Lower dilution factors will be used in order to reduce the log difference between rows. Changing the irradiance levels will also help to improve the accuracy of the titre.

 

Reference: Bacteria survival is done by counting the number of live cultures after an incubation period. Virus cells can not be counted to determine survival so a desirable endpoint that represents biological quantization is one where 50% of the virus inoculated cells show a cytopathic effect (CPE). CRFK feline kidney cells show a dramatic change in appearance when infected with FeCV. TCID50 represents this effect in inoculated cultures where TCID stands for "tissue culture infectious dose". A series of dilutions of the treated virus are prepared and inoculated onto normal growing animal cells in replicate wells on a multi-well plate with 8 rows of 6 columns. After the appropriate incubation time, the cultures are scored for positive or negative CPE. This scoring is used to calculate a titre based on a formula for limiting dilution by Karber. Critical to the use of this formula are the highest dilution giving 100% CPE which did not occur with this test, the dilution interval and the well positive proportions per dilution.

GermBuster™ under a biosafety cabinet
 

Typical setup for Sterilray™ under a biosafety cabinet.

 

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